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Description

List Price

£4504

Gradient Making Equipment - Biocomp

The performance of its two elements, the Piston Gradient Fractinator™ and the Gradient Master™ is identical to the separate instruments.

Features: The Gradient Station combines all the great features of the Gradient Master and the Piston Gradient

Gradient Master - see product 108-2

This is biocomp's premier gradient forming instrument.
in as little as 40 sec, you can produce 6 identical, linear gradients using a patented process we call tilted tube rotation. The process involves layering the two end point solutions (i. E. 5% and 30% sucrose) directly in the centrifuge tube, capping it and placing it in a special tube holder with a magnetic base (magnabase™) that adheres to a steep plate on the instrument. Two keystrokes later, the gradient master takes over and tilts the tube to the proper angle, rotates it for a designated time and returns it to its original vertical position.

the introduction of the model 107 is the latest step in the evolution of this remarkable instrument. It has several new features that distinguish it from previous models. First, its memory contains all the run parameters for all the many different gradients we have developed for our customers. To see the list, select the rotor (ie sw41) you are using and then scroll through a list of the gradients organized by % and solute. To simplify things further, the “last” key on the keyboard recalls the last gradient run, so 2 keystrokes are all that are needed to duplicate a run.
second, the unit is now internet programmable. You can download new software yourself as upgrades become available. The gradient catalog is also upgradable so users can benefit from our ever-expanding set of run parameters. Solutes used so far include sucrose, glycerol, optiprep®, nycodenz®, ficoll®, percoll®, metrizamide, renografin®, nacl, cscl, naac, kcl. Like the old saying, the gradient master has never met a solute it didn’t like.

the gradient master is also a patented, intelligent roller blot device that can perform all the blocking, blotting and reaction steps with unerring accuracy and reproducibility. Using our patented magnabase™ bottles which come in a variety of sizes to fit every blot without overlap, you can reduce your reagent use by 90% while obtaining superb blots.
if you are serious about your gradient work, there is no competition for the gradient master. In nearly a thousand labs around the world for 18 years, it has become the gradient former of choice because of its simplicity, incredible speed and durability. features:the base unit includes a backlit lcd display, control knob and "smart" keypad for data entry and control. A 6" steel plate is tilted and turned by two stepper motors. The kit includes mac and pc serial cables for programming the unit, a bubble level, two 4” cannula for layering of gradient solutions and a selection of allen wrenches for maintenance.

Gradient Fractionator - see product 152-002

Developed by a scientist who routinely does 6-30 gradient experiments, this device has more performance-enhancing features than all competing designs combined. You can recover slices from the gradient as small as 10 microns thick (300 micron fractions are more typical), visualize bands of particles as small as ribosomes with the naked eye, fractionate manually to recover visible bands or automatically to produce perfect gradient profiles, and rinse and blow dry your sample tubing after each fraction, eliminating cross-contamination between fractions.
the most important technical advance is our patented “trumpet tip”™, an inverted tapered piston cone that gradually compresses bands of particles from horizontal discs into thin vertical columns. This advance greatly reduces the resolution-killing laminar capillary flow that tends to pull sample from a teardrop shaped area directly in front of the needle or cone used by our competitors.

Another significant improvement is the readout of distance (0. 00 mm) from the meniscus down, so you know exactly where you are sampling in the tube.
the results are nothing short of spectacular. In a recent paper, jardine & coombs (1998) resolved 4 distinct peaks of phage t4 capsids between 300s - 350s where previous reports fractionated by needle puncture had shown only one broad peak. One of the new particles was a new intermediate in head assembly that demonstrated that capsid expansion followed the beginning of dna packaging.

The combination of the gradient master and the piston gradient fractionator offer a quantum leap in reproducibility and resolution. Call today for details. features: • visible light scattering band visualization system for particles as small as viruses and ribosomes.