cole-parmer-techne

Techne® is a long established name and world leader in the manufacture of temperature control and essential equipment for the life sciences, research, clinical, general laboratories, calibration and industrial applications. The lab products provided by Techne are the leading in cutting edge temperature control technology.

Authorised dealer for:

UK only

30/06/2021

Cole-Parmer - Techne thermal cycler from Wolflabs

Product Update

Introduction
Cole-Parmer - Techne 3PrimeX thermal cycler from Wolflabs.

Features and benefits
- Colour touchscreen interface for fast programme set up.
- Compact unit.
- Easy to use.
- Internal memory can hold 1000 programmes.
- Data transfer via USB.
 

Product Category: Thermal Cyclers

Manufacturer: Cole-Parmer - Techne

24/08/2020

Cole-Parmer - Techne thermal cycler from Wolflabs

Product Update

Introduction
Cole-Parmer - Techne 3PrimeX thermal cycler from Wolflabs. The 3PrimeX delivers all the features of the ³Prime instrument but with an expanded sample capacity, accommodating 48 x 0.2ml microtubes, 30 x 0.5ml tubes plus half a 96 well plate in a horizontal format. Design flexibility allows the unit to be upgraded to include gradient cycling capability.

Features and benefits
- Colour touchscreen interface for fast program setup.
- Small space-saving footprint.
- Fast ramp rate up to 3.0°C/sec.
- Data Transfer via USB.

Product Category: Thermal Cyclers

Manufacturer: Cole-Parmer - Techne

17/09/2019

Cole-Parmer - Techne stirred water bath from Wolflabs

Product Update

Introduction
Cole-Parmer - Techne TU-20D + FBATH18 stirred water bath from Wolflabs. 

Features and benefits
- Stainless stell construction.
- Integrated carrying handle.
- 3 year warranty.
- Rugged splash-proof case.

Product Category: Water Baths - Stirred

Manufacturer: Cole-Parmer - Techne

10/07/2019

Cole-Parmer - Techne thermal cycler from Wolflabs

Product Update

Introduction
Cole-Parmer - Techne 5PRIME/C thermal cycler from Wolflabs. This thermal cycler provides unrivalled flexibility with ability to accommodate 33 x 0.2ml tubes and 33 x 0.5ml tubes, in 6 rows and 11 columns. Prime CombiBlock cyclers are available with or without a temperature gradient, which is upgradable later.

Features and benefits
- Total reliability.
- Exceptional value for money.
- Colour touchscreen for fast program setup.

Product Category: Thermal Cyclers

Manufacturer: Cole-Parmer - Techne

10/03/2018

Cole-Parmer - Techne block heater from Wolflabs

Product Update

Introduction
Cole-Parmer - Techne DB-2A block heater from Wolflabs. Techne's Dri-block heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

Features and benefits
- Small, light and compact footprint, economical price
- Can hold up to 2 aluminium insert blocks or one 96-well plate block
- Analogue: temperature setting is by a calibrated dial
- Temperature range from ambient to 100°C or 200ºC
- Fast heat-up rate from 30°C to 100°C in just 12 minutes
- Temperature stability at 40°C: ± 0.05°C

Product Category: Block Heaters

Manufacturer: Cole-Parmer - Techne

Thermal Cyclers - Techne
 

 

 


 

 

 

 

48-Well Thermal Cyclers

TC-3000G

The world's smallest gradient cycler, the TC-3000G will hold half a 96-well plate, in a horizontal format so that it offers 8 columns for annealing temperature optimisation and 6 rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over a 15°C gradient between the temperatures of 20 and 80°C.

All the features of the TC-3000 and TC-3000X plus...

•Gradient block: A gradient of up to 15°C between the temperatures of 20 and 80°C can be set, allowing any protocol to be optimised within a single experiment.
•New software: Enables a gradient to be added to any temperature step of the program.
•Gradient calculator: The gradient calculator function displays the temperature for each of the 8 columns, ensuring easy replication of thermal conditions.

Full Size 96-well Thermal Cyclers

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and ideal for a larger number of parallel samples. Both units offer:

•Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
•Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
•Temperature range of 4°C to 99°C
•8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
•Incremental/decremental time and temperature for applications such as Touchdown PCR
•The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software

TC-5000

The TC-5000 gradient thermal cycler enables you to optimise your experiments at the `touch of a screen', with the same mechanical features as the TC-4000.

•Real-time' graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment's status.
•User-friendly programming interface provided by a 115 x 90mm touch-sensitive screen.
•Excellent heating rate: A maximum heating rate of 3°C/sec and block uniformity of ±0.3°C at 50°C with or without a gradient...no compromise!
•The most linear gradient cycler on the market, with an amazing range of 30°C. 
•The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.
•Gradient range 20°C to 70°C, maximum 30°C
•Decremental/incremental time and temperature

TC-Plus Thermal Cyclers

The TC-PLUS thermal cyclers have all the latest features from a colour touchscreen to fast ramp rates along with some innovative additions such as an automatic, non-motorised heated lid, TERS®, thermal energy recovery system and a unique space-saving, stackable design. The TC-PLUS and Satellite units can be combined to form any multi-block format with the Satellites being controllable from either a PC or the TC-PLUS.

Key Features

•Colour touchscreen for fast program setup.
•TERS®, Thermal Energy Recovery System to save on energy consumption.
•Unique, easy to use CD-type sample drawer mechanism which provides the correct pressure for use with caps: domed or flat, heat OR adhesive seals without the use of a motor!
•Space-saving, stackable design to save on bench space!
•Satellite option which can be controlled by either the TC-PLUS or a PC using Techne Workbench software.
•Fast ramp rate of up to 5°C/sec.
•Three gradient block options; 96 x 0.2ml, 60 x 0.5ml and 384-well.
•Tm calculator, which uses the Nearest Neighbour algorithm.
•Backed by Techne's 4 year warranty.*

*4 year for the TC-PLUS thermal cycler and 80,000 cycles or 4 year warranty for the thermal block, whichever comes first.

Technical Information

Model: TC-3000G TC-5000 TC-PLUS
0.5ml block capacity 30 60 60
0.2ml block capacity 48 96 96
384-well block NA Yes Yes
In-situ block NA Yes NA
Temperature range 4°C to 99°C 4°C to 99°C 0 to 100° C
Gradient range 20°C to 80°C 20°C to 70°C Across the full temperature range
Maximum gradient1 15°C 30°C (16°C for 384 block) 30° C
Minimum gradient NA NA 1° C
Pre-run sample cooling NA NA Yes at 4° C
Maximum ramp rate 3.3°C/sec 3.0°C/sec NA
Block uniformity at 50°C ±0.2°C <±0.3°C  (without gradient) ± 0.3° C at 50° C
Temperature accuracy NA NA ± 0.2° C at 50° C
Maximum heating rate NA NA 5° C/ s
Maximum cooling rate NA NA 3.0° C/ s
Peltiers per block 6 NA NA
selectable heated lid temperature 100°C to 115°C 100°C - 115°C 35 to 115° C or off
Heated lid enable/disable Yes Yes NA
Over-temperature cut-out NA Yes Yes
Regulated heated lid pressure NA NA Automatic
No. of programs 80 50 1000
Programming display 4-line alphanumeric Touchscreen 5.7" VGA colour touchscreen
Graphical display NA Real-Time Graph graphical display
Maximum hold time NA 99 hrs 99h 59m 59s
Minimum hold time NA 1 sec NA
Gradient calculator NA NA Yes
Incrementaldecremental temperature NA NA Yes
Incrementaldecremental time NA NA Yes
Password protection NA NA Yes
Auto restart on power failure Yes Yes Yes with options of 10min 30min 1h or always
Connection to PC control software NA NA Yes
Tm primer calculator NA NA Yes
Dimensions L x W x H 330 x 170 x 190mm 420 x 220 x 260mm 375 x 276 x 312mm
Voltage Dual voltage 230/115V/100V 50-60Hz 230/115V 50-60Hz 00-230V 50-60Hz
Power 230W 620W 950W
Shipping weight 6kg 14kg 14kg

 

Thermal Cyclers - Techne
 

Full Size 96-well Thermal Cyclers

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and ideal for a larger number of parallel samples. Both units offer:

• Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
• Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
• Temperature range of 4°C to 99°C
• 8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
• Incremental/decremental time and temperature for applications such as Touchdown PCR
• The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software

TC-4000

The TC-4000 is one of the most affordable full sized thermal cyclers in the market, flexible for all your protocols and easy on your budget.

•Intuitive programming: 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.
•Excellent heating rate of 2.6°C/sec and block uniformity of ±0.3°C at 50°C, ensuring optimal reproducibility
 

Technical Information

Model: TC-4000
0.5ml block capacity 60
0.2ml block capacity 96
384-well block Yes
In-situ block Yes
Temperature range 4°C to 99°C
Gradient range NA
Maximum gradient1 NA
Maximum ramp rate2 2.6°C/sec
Block uniformity at 50°C <±0.3°C
selectable heated lid temperature 100°C - 115°C
Heated lid enable/disable Yes
Over-temperature cut-out Yes
No. of programs3 80
Programming display 4-line alphanumeric
Graphical display NA
Maximum hold time 18 hrs
Minimum hold time 1 sec
Auto restart on power failure Yes
Dimensions L x W x H 420 x 220 x 260mm
Voltage Dual voltage 230/115V 50-60Hz
Power 620W
Shipping weight 13kg

 

 

Thermal Cyclers - Techne

48-Well Thermal Cyclers

TC-3000X

The new TC-3000X personal cycler is based on the unrivalled and reliable TC-3000. The new model is ideal for those laboratories that need just that little bit more, with its expanded sample capacity to accommodate 48 x 0.2ml tubes or for convenience, a 48-well plate.

• Increased sample capacity: The footprint remains the same, only 561cm2,but the capacity increases to 48 x 0.2ml or 30 x 0.5ml tubes
• Adjustable heated lid: The heated lid can now be adjusted for use with all PCR tubes, strip tubes or 48-well plates sealed with sealing mats
• Rapid heating rate: Utilising the latest Peltier technology the TC-3000X has a heating ramp rate of 3.3°C/sec and a 2.0°C/sec cooling rate
• Fast-track programming: Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple
• PC control & networking: The TC-3000X can be linked together with other Techne cyclers; up to 32 cyclers can be networked to one computer
 

Technical Information

Model: TC-3000X
0.5ml block capacity 30
0.2ml block capacity 48
Temperature range 4°C to 99°C
Maximum ramp rate 3.3°C/sec
Gradient range NA
Maximum gradient NA
Block uniformity at 50°C ±0.2°C
Peltiers per block 6
selec table heatd lid temperature 100°C to 115°C
Heated lid enable/disable Yes
No. of programs 80
Programming display 4-line alphanumeric
Auto restart on power failure Yes
Dimensions L x W x H 330 x 170 x 190mm
Voltage 230/115V/100V 50-60Hz
Power 230W
Shipping weight 6kg

 

Thermal Cyclers - Techne
 

TC-3000 Personal 25-well Thermal Cycler


The TC-3000 has been designed with research and teaching laboratories in mind, the TC-3000 offers the ultimate in low cost solutions where ease of use is high on the priority list. Experience in thermal cycler manufacturing since 1987, ensures that the TC-3000 provides guaranteed performance and quality results, every time.

•Space saving small footprint: at only 561cm2, the TC-3000 is even economical on space
•Rapid heating rate with a 3.6°C/sec heating rate and 2.0°C/sec cooling rate 
•Adjustable heated lid (100°C to 115°C), designed to reduce the risk of sample evaporation, can be enabled or disabled. The heated lid only comes on if the block is set above 35°C 
•Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple 
•The TC-3000 can be linked together with up to 32 cyclers can be networked to one computer
 

Technical Information

Model: TC-3000
0.5ml block capacity 20
0.2ml block capacity 25
Temperature range 4°C to 99°C
Maximum ramp rate 3.6°C/sec
Block uniformity at 50°C ±0.1°C
Peltiers per block 4
selec table heatd lid temperature 100°C to 115°C
Heated lid enable/disable Yes
No. of programs 80
Programming display 4-line alphanumeric
Auto restart on power failure Yes
Dimensions L x W x H 330 x 170 x 190mm
Voltage Dual voltage 230/115V 50-60Hz
Power 230W
Shipping weight 8kg

 

 

Touchgene Thermal Cycler

 

The Techne Touchgene Gradient is the only Gradient thermal cycler which enables you to optimise your experiments at the “touch of a screen.”

o 4-Fold control: Each block consists of 8 peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block. This ensures that the Touchgene Gradient exhibits the most accurately controlled linear gradient available*.
 
o High performance: A maximum heating rate of 3°C/Sec and block uniformity of <±0.4°C. With or without a gradient......no compromise!
 
o Versatile block format: The truly user-friendly interchangeable block system allows blocks to be interchanged in a matter of seconds without the need of any tools (60 x 0.5ml 96 x 0.2 ml, 3841 well, Insitu Block2).
 
o Proven track record: Having more than 13 years experience in thermal cycler design ensures that the Techne Touchgene Gradient encompasses reliability with all the specifications you could wish. These include: PC control and networking, decremental/incremental time and temperature, and defined pressure programmable heated lid.
 
o Intuitive programming: Easy to use software with free upgrades downloadable from the Techne website as new functionality is added.
 
o New Design Flexible Heated Lid: Defined pressure, high quality, programmable heated lid to accommodate a variety of consumables..
Wide linear gradient
The most linear gradient cycler on the market, with an amazing range of 30°C. Protocols can be optimised in a single experiment.
 
TouchScreen
The Touchgene Gradient incorporates a large, 115 x 90mm, touch-sensitive screen that provides a unique user-friendly programming interface which is second to none.
 
Graphical display
The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment’s status.The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment’s status.
 
o Memory cards: SmartMedia memory cards provide an alternative to internal memory storage of your programs, offering security and flexibility.
 
o Gradient calculator: The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.

Touchgene Thermal Cycler Specifications

SPECIFICATION

BLOCK TEMPERATURE

Gradient  range 20ºC to 70ºC
Maximum gradient 30ºC
Minimum gradient 1ºC
Number of temperature probes 4
Block temperature range 4ºC to 99ºC
Max. ramp rate* 3.0ºC/sec
Block uniformity without a gradient* <±0.4ºC
Column uniformity with a 10ºC gradient* <±0.3ºC
Temperature accuracy*  <±0.3ºC
Gradient accuracy* <±0.3ºC
Temperature set point adjustment 0.1ºC
Heated Lid
Selectable heated lid temperature  100ºC - 115ºC
Heated lid enable/disable Yes
Warm up time ambient (to 105ºC) 4 min
Over temperature cut out Yes
Regulated lid pressure Yes
Heated lid only comes on if block is set above 30º  
Programming
Password protection Yes
Incremental / decremental temperature Yes
Incremental / decremental temperature segment hold time Yes
Variable programmable ramp rate steps ±0.1ºC/sec
Programmable ramp rate resolution 0.1ºC
Gradient calculator Yes
Maximun programs internal memory 50#
Maximun programs memory card 500#
Program name capability Alphanumeric
Programming interface (115x90 mm screen) Touch Sensitive
Pause facility Yes
End of program alarm (can be disabled) Yes
Auto restart on power failure Yes
Run end time calculation Yes
Miscellaneous
Dimensions L x W x H (mm) 420 x 220 x 260
Net weight 11.5kg
Serial port RS 232 / RS 485 Yes
Connection to PC control program  Yes
Free software upgrades from website Yes
Power consumption 620 Watts
Ambient temprature range 5ºC to 40ºC
Relative humidity tolerance up to 95%
Frequency 50 - 60 Hz
Voltage 100v or 120v/230v 


*specifications given are typical values in ambient temperature of 20 ºC for non-gradient (unless stated).
#Actual numbers dependent on complexity of the programs. 

Progene Thermal Cycler

SPECIFICATION

TEMPERATURE

Temperature range°C 4 to 99
Temperature control precision°C ±0.1
Block uniformity°C ±0.3
End of program cooling below ambient Yes

PROGRAMMING

Number of programs 50 (3 segment programs)
Maximum number of segments/program 9
Maximum number of repeat cycles per program 99
Program to program linking Yes
End of program alarm Yes
Pause button Yes
Incremental/Decremental timing second intervals
Incremental/Decremental temperature 0.1°C to 5.6°C in 0.1°C intervals
Programmable ramp rate - up........ Programmable ramp rate - down... variable from 1°C to 60°C/minute

HEATING/COOLING RATE

25x0.2ml micro tube/5x5 multiwell plates Cooling 2.2°C /sec. Heating 2.8°C /sec.
20x0.5ml microcentrifuge tubes Cooling 2.0°C/sec. Heating 2.5°C/sec.
Dimensions LxWxH (mm) 350x155x173

NET WEIGHT 6kg

 

Progene offers all the following features:
Universal heated lid with unique clamping mechanism for oil free PCR 50/60 cycles
Interchangeable blocks Incremental/decremental timing
Minimum temperature 4°C Incremental/decremental temperature programming
Parallel printer port Manual pause
RS232/PC link to store print profile information Cycle count
Auto restart Two year warranty
Voltage 90/132V 210/260V

 

Progene Thermal Cycler

 

 

 

Smallest in the World*

  • Fastest in the World*
  • Least expensive in the world*
  • Nothing is extra - an amazing list of innovative features as standard and with "user friendly" software that can accommodate all the latest PCR protocols such as "Touchdown", "RAPD", and "Long PCR".
  • Unrivaled performance - better than 2.2°C cooling 2.8°C heating per second in the 55°C to 90°C range - dependent on block type.
  • Temperature range 4°C to 99°C
  • Heated lid for oil free PCR
  • Two models available for 25 x 0.2ml micro tubes/ 5x5 multiwell plate and 20 x 0.5ml microcentrifuge tubes
  • Full two year warranty
 

Progene has numerous applications in Molecular Biology and Diagnostic laboratories and is suitable for use with diagnostic kits.
Progene has been designed for the individual; priced to be inexpensive enough to be treated as an ordinary piece of laboratory equipment that everybody can own and designed to process a small number of parallel samples rapidly in unprecedented cycle times, allowing a typical 25 cycle program to be completed in around an hour, enabling the operator to achieve more PCR's in a working day.
Using advanced Peltier technology gathered from Techne's larger Cyclogene thermal cycler and developed to give the amazing heating/cooling rates of better than 2.2 - 2.8°C/second (average cooling and heating) and using a high tolerance PRT sensor to give a temperature stability of ±0.1°C and a block uniformity of ±0.3°C.
Progene is available with heated lid as standard and a specification only sometimes found in units costing two or three times the Progene price.
One of Progene's many features is incremental/decremental segment hold time. This allows the user to increase or decrease the hold time of a given segment hold with each cycle. Use incrementally on 'extension' for techniques such as Long PCR and RAPD to allow sufficient time for the increasing quantity of DNA to extend. Use decrementally on 'denaturation' to allow initial highly specific denaturation and then less time with each cycle at 95°C so as to maintain the enzyme's (taq) half-life. Additionally, another feature of Progene is incremental/decremental temperature used for protocols such as Touchdown PCR to optimise annealing. The interchangeable blocks available (to suit 0.5ml microcentrifuge tubes, 0.2ml micro tube/5x5 multiwell plate) can be changed in seconds.
Progene is small enough and inexpensive enough to be personal but with its technically innovative, superior robust specification, reliability and performance capabilities, suitable for the most demanding molecular scientist.

GENIUS Thermal Cycler Specifications

 

SPECIFICATION

TEMPERATURE

Temperature range
Temperature control precision
Block uniformity 
4°C to 99°C
±0.2°C
±0.5°

PROGRAMMING

End of program cooling below ambient
Number of programs
Maximum number of segments per program
Maximum number of repeat cycles per program
Program to program linking
End of program alarm
Pause button 
Yes
99
9
99
Yes
Yes
Yes
Yes 
Incremental/Decremental timing 1 second intervals 
Incremental/Decremental temperature 0.1°C to 5.6°C in 0.1°C intervals 
Programmable ramp rate - up........
Programmable ramp rate - down... 
variable from 1°C to 60°C/minute

HEATING/COOLING RATE

96x0.2ml micro tube Cooling 1.6°C /second
Heating 2.6°C /second 
40 x 0.5ml microcentrifuge tubes Cooling 1.8°C/second
Heating 2.8°C/second
Dimensions L x W x H (mm) 420 x 220 x 240 

NET WEIGHT 10kg

 

GENIUS Thermal Cycler Computer Connectivity Features

Computer Communications Via RS232 port 
Using a software package such as 'PROCOMM' or Microsoft Windows 'TERMINAL' the Genius can be configured to carry out the following functions: 

  1. Store print profile information 

  2. Select a program number 

  3. Start and stop a selected program 

RS232 Software 
The Techne software package GENSOFT is available as an accessory for connecting the Genius to computer via the RS232 port. The software incorporates some additional features making the unit even more flexible for those who have larger throughput of samples normally requiring more than one machine or where a unit is required to interface with a robotics system. 
These features include 

  1. Select, start and stop a program. 
  2. Daisy chaining a number of units together to a maximum of 32. 
  3. Creation of thermal cycling protocols through an easy to use Microsoft Windows style user interface and transmittable to one or more units. 
  4. Storage of protocols in named directory. 
  5. Diagrammatic status of all units connected including information on the current position in protocol of each individual unit and time remaining. 
  6. Print-out of protocols in a diagrammatic format together with associated notes page. 

GeniusQuad Package
A reduced price package containing all you require for high throughput cycling with the option to connect through a PC:

o 4 x Genius cyclers in 96 or 384 microtitre plate formats
o 1 x Genius control software
o 1 x Computer connector cable with RS232: RS458 converter, to the first cycler to a PC
o 4 x Genius connector boxes and connection cables to PC-enabled each cycler
o 4 x Genius cyclers in 96 or 384 microtitre plate formats
Safe: No exposure to boiling agarose and reduced handling of carcinogenic Ethidium Bromide.

Genius Thermal Cycler

 

 

 

 

The new Genius Thermal Cycler has been designed for the laboratory that has a high throughput requirement of samples. A wide selection of block options up to and including a 384 well microplate makes Genius the number one choice for large scale screening and genome investigations. 

Regardless of the block type being used all samples are heated to a programmed temperature and held to very precise limits. 

Speed - Throughput of samples is quick - due to overall performance of the heating and cooling cycle of the Geniusa 30 cycle program can be completed in well under one hour, freeing the instrument for several PCR runs in a normal working day. 

Performance - In addition to the speed of heating and cooling the on board processor PRT senior ensure a high degree of temperature stability and uniformity of the block so that all samples are processed to the programmed requirements. 

The Genius Thermal Cycler comes complete with a heated lid as standard and is designed to reduce to a minimum refluxing of the sample onto the lid and side walls of the sample tube. 

Flexigene/Flexigene Quad Thermal Cyclers

The Flexigene is a high performance, high sample throughput instrument at a highly affordable price.

Flexible block format: The truly user-friendly fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need of any tools (60 x 0.5ml, 96 x 0.2ml, 96 x 0.2ml for fully skirted plates, 384-well or flat plate for in-situ).

New design flexible heated lid: Defined pressure, programmable heated lid to accommodate a variety of consumables.

Intuitive programming: New 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.

8 Peltiers: Building on the innovative Quad Circuit Technology of the Touchgene Gradient, the Flexigene ensures unsurpassed control accuracy and temperature uniformity.

Low cost: The Flexigene is probably the most affordable full sized Thermal Cycler.

PC networking: The Flexigene can be connected to a PC along with other Techne cyclers for low volume or high throughput applications using Gensoft*.

 

Flexigene Thermal Cycler Specifications

Specifications

Blocks Formats

0.5ml capacity 60
0.2ml capacity 96
384-well block Yes
In-situ block Yes
User interchangeable Yes

Block Temperature Gradient

Maximum ramp rate* 2.6°C/sec
Block uniformity at 50°C* <±0.3°C
Temperature range 4°C to 99°C
Temperature set point precision 0.1°C
Heated Lid
Selectable heated lid temperature 100°C - 115°C
Heated lid enable/disable Yes
Over temperature cut out Yes
Regulated lid pressure Yes
Heated lid only comes on if block is set above 35°C
Programming
Number of programs # 80
Password protection Yes
Programming 4-line alphaN
Graphical display No
Incremental/decremental temperature Yes
Incremental/decremental hold time Yes
Maximum hold time 18 hrs
Minimum hold time 1 sec
Variable programmable ramp rate 0.1°C/sec steps
Run end time calculation Yes
Pause and stop facility Yes
End of program alarm (enable/disable) Yes
Auto restart on power failure Yes
Miscellaneous
Serial port RS232
Dual Voltage (120/230V) Yes
Temperature sensor Thermistor
Peltierelements/block 8
Dimensions L x W x H (mm) 420 x 220 x 260
Footprint L x W (mm) 420 x 220
Connection to PC control program Yes
Free software upgrades from website Yes
Power consumption 620 Watts
Frequency 50 - 60 Hz

Net weight

11kg (total)

 

 

Techgene Thermal Cycler


Small, low cost
Out of the worlds best selling thermal cyclers, the Techgene is the smallest, lowest cost unit on the market. It is available with 25 x 0.2 ml or 20 x 0.5 ml microtube interchangeable block formats.

Fast-track programming with free software upgrades
With modern, intuitive programming and ready-to-go templates, creating even the most complicated protocols is simple.

As new functionality is added to the software, updates can be downloaded for free from the Techne website.

Reliable, high performance
Experience in thermal cycler manufacturing since 1987, ensures that the Techgene provides both guaranteed performance and quality results, everytime.

Portable Dual Voltage
This Compact unit is not only light, but has a variable voltage selector so you can take it with you anywhere in the world!

Key Features:

o

Unparalleled block uniformity: ±0.1ºC at 50 ºC, ensuring optimal reproducibility

o

Fastest heating rate: up to 3.6ºC/Sec heating rate and 2.0ºC/Sec cooling rate

o

Smallest footprint yet: at only 561 cm2, the Techgene is even economical on space

o

PC control & networking: The Techgene can be networked together with other Techne cyclers allowing up to 32 cyclers to be connected to one PC.

o

Password protection: Protect your program against unwanted modifications.

o

Heated lid: The selectable temperature range heated lid (+100ºC to +115ºC) is sprung to fit a variety of height tubes and can be enabled or disable

o

Full 2 year warranty

 

Flexigene Thermal Cycler

The new Flexigene thermal cycler, flexible on your protocols, easy on your budget.

The Flexigene is a high performance, high sample throughput instrument at a highly affordable price.

Flexible block format: The truly user-friendly fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need of any tools (40 x 0.5ml, 96 x 0.2ml, 96 x 0.2ml for fully skirted plates, 384-well or flat plate for in-situ).

New design flexible heated lid: Defined pressure, programmable heated lid to accommodate a variety of consumables.

Intuitive programming: New 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.

8 Peltiers: Building on the innovative Quad Circuit Technology of the Touchgene Gradient, the Flexigene ensures unsurpassed control accuracy and temperature uniformity.

Low cost: The Flexigene is probably the most affordable full sized Thermal Cycler.

 

PC networking: The Flexigene can be connected to a PC along with other Techne cyclers for low volume or high throughput applications using Gensoft*

* Up to 32 Techne cyclers can be connected and run through a PC using Gensoft,
a software package which can be downloaded free of charge from their website.

 

FlexiQuad Package

Unbeatable value

This reduced price package containing all you require for high throughput cycling with the option to connect through a PC:

  • 4 x Flexigene cyclers in 96 standard, 96 fully skirted or 384 well microtitre plate format.
  • Gensoft control software (also free of charge from the our website).
  • 1 x computer connector cable with RS232:RS485 converter, to connect the first cycler to a PC
  • 4 x Flexigene connector boxes and connections.
  • 1 x termination box with power pack and power cable, to place on the last cycler.
 

Specifications

Blocks Formats

0.5ml capacity 40
0.2ml capacity 96
384-well block Yes
In-situ block Yes
User interchangeable Yes

Block Temperature Gradient

Maximum ramp rate* 2.6°C/sec
Block uniformity at 50°C* <±0.3°C
Temperature range 4°C to 99°C
Temperature set point precision 0.1°C
Heated Lid
Selectable heated lid temperature 100°C - 115°C
Heated lid enable/disable Yes
Over temperature cut out Yes
Regulated lid pressure Yes
Heated lid only comes on if block is set above 35°C
Programming
Number of programs # 80
Password protection Yes
Programming 4-line alphaN
Graphical display No
Incremental/decremental temperature Yes
Incremental/decremental hold time Yes
Maximum hold time 18 hrs
Minimum hold time 1 sec
Variable programmable ramp rate 0.1°C/sec steps
Run end time calculation Yes
Pause and stop facility Yes
End of program alarm (enable/disable) Yes
Auto restart on power failure Yes
Miscellaneous
Serial port RS232
Dual Voltage (120/230V) Yes
Temperature sensor Thermistor
Peltierelements/block 8
Dimensions L x W x H (mm) 420 x 220 x 260
Footprint L x W (mm) 420 x 220
Connection to PC control program Yes
Free software upgrades from website Yes
Power consumption 620 Watts
Frequency 50 - 60 Hz

Net weight

11kg (total)

* Given are typical values of a standard 0.2ml block, in an ambient temperature of 20°C.
# Actual numbers are dependent on complexity of the programs. All Techne cyclers have a full 2 year Warranty.

Technical notes:
(REF FGEN-01)

Block temperatures
To provide thermal cyclers with the high performance and specificity required for the protocols of today, heated block design and program versatility are important features.

Incremental/decremental temperatures
Touchdown PCR protocols are where a higher temperature is required for the initial cycles than later cycles. This ensures that only highly specific product is created (amplified) during the critical initial steps. A decreased, less stringent temperature is then used by gradually decreasing the annealing temperature. The effect is a higher yield and more specific product. Therefore, to eliminate the need for programming lengthy error prone protocols, choose a thermal cycler which can automatically incorporate an incremental decrease/increase in temperature.

Incremental/decremental time
Long template PCR is the amplification of large fragments of DNA (e.g. 40kb lambda, 27kb Genomic). To ensure that the extension is fully complete after each cycle, the extension time is increased with each cycle to compensate for the increased product quantity and decreasing concentrations of reagents. As above with temperature, choose a thermal cycler which can automatically increase/decrease hold time at a given temperature.

Peltier technology 
The peltier effect is used in many applications for the reliable cooling of sensitive high-temperature components without the need for refrigerants or compressors. Otherwise known as thermoelectric coolers, peltiers are a special type of semiconductor that function as a solid state heat pump. By applying a low voltage DC power source to one side, heat will be moved in the direction of the current (+ to - ). The heat is thus pumped from one side of the module to the other, so that one face will be cold while the opposite face will be heated. As this effect is reversible, peltier technology is the chosen method for heating and cooling the block in the best thermal cyclers on the market today.


Thermal cycler Techne TC-PLUS gradient

       

     

The TC-PLUS thermal cyclers have all the latest features from a colour touch screen to fast ramp rates along with some innovative additions such as an automatic, non-motorised heated lid (patent pending), TERS™, thermal energy recovery system (patent pending) and a unique space-saving, stackable design. The TC-PLUS and Satellite units can be combined to form any multi-block format with the Satellites being controllable from either a PC or the TC-PLUS. 

Key Features
• Colour touch screen for fast program setup
• TERS™, Thermal Energy Recovery System (patent pending) to save on energy consumption
• Unique, easy to use CD-type sample drawer mechanism (patent pending) which provides the correct pressure for use with caps: domed or flat, heat OR adhesive seals without the use of a motor!
• Space-saving, stackable design to save on bench space!
• Satellite option which can be controlled by either the TC-PLUS or a PC
• Fast ramp rate of up to 5oC/sec
• Three gradient block options; 96 x 0.2ml, 60 x 0.5ml and 384-well
• Tm calculator, which uses the Nearest Neighbour algorithm
• Backed by Techne's 4 year warranty*


Technical Specification

Sample capacity: 0.2ml 96
Sample capacity: 0.5ml 60
Sample capacity: 384-well Yes
Block temperature range 0 to 100°C**
Block uniformity (without gradient) <±0.3°C
Temperature accuracy <±0.2°C
Gradient range Across the full temperature range
Maximum gradient 30°C
Minimum gradient 1°C
Pre-run sample cooling Yes at 4°C
Maximum heating rate 5°C/s
Maximum cooling rate 3.0°C/s
Selectable heated lid temperature 35 to 115°C or off
Over-temperature cut-out Yes
Regulated heated lid pressure Automatic
Program interface 5.7" VGA colour touchscreen with graphical display
Maximum number of programs stored 1000
Maximum hold time 99h 59m 59s
Gradient calculator Yes
Incremental/decremental temperature Yes
Incremental/decremental time Yes
Password protection Yes
Auto re-start on power failure Yes with options of 10min, 30min, 1h or always
Connection to PC control software Yes
Tm primer calculator Yes
Dimensions (L x W x H) 375 x 276 x 312mm
Weight 14kg
Voltage 100-230V, 50-60Hz
Power 950W

 


Ordering Information

Product Code Description
FTCPLUS/02 TC-PLUS thermal cycler, 96 x 0.2ml
FTCPLUS/05 TC-PLUS thermal cycler, 60 x 0.5ml
FTCPLUS/384 TC-PLUS thermal cycler, 384-well plates
FTCSAT/02 TC-PLUS satellite thermal cycler, 96 x 0.2ml
FTCSAT/05 TC-PLUS satellite thermal cycler, 60 x 0.5ml
FTCSAT/384 TC-PLUS satellite thermal cycler, 384-well plates
FTCPLUS/02/B TC-PLUS block, 96 x 0.2ml
FTCPLUS/05/B TC-PLUS block, 60 x 0.5ml
FTCPLUS/384/B TC-PLUS block, 384-well plates











 

 

* 4 year for the TC-PLUS thermal cycler and 80,000 cycles or 4 year warranty for the thermal block, whichever comes first.

Purchase of this instrument conveys a limited, non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC

 

Thermal cycler Techne TC-4000 non gradient and TC-5000 gradient/non gradient

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and ideal for a larger number of parallel samples. Both units offer:

• Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
• Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
• Temperature range of 4°C to 99°C
• 8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
•Incremental/detrimental time and temperature for applications such as Touchdown PCR
• The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software
 

Thermal cycler Techne TC-4000 non gradient

TC-4000

The TC-4000 is one of the most affordable full sized thermal cyclers in the market, flexible for all your protocols and easy on your budget.

•Intuitive programming: 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.
•Excellent heating rate of 2.6°C/sec and block uniformity of ±0.3°C at 50°C, ensuring optimal reproducibility

 

Thermal cycler Techne TC-5000 gradient/non gradient

The TC-5000 gradient thermal cycler enables you to optimise your experiments at the `touch of a screen', with the same mechanical features as the TC-4000.


• `Real-time' graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment's status.
•User-friendly programming interface provided by a 115 x 90mm touch-sensitive screen.
•Excellent heating rate: A maximum heating rate of 3°C/sec and block uniformity of ±0.3°C at 50°C with or without a gradient...no compromise!
•The most linear gradient cycler on the market, with an amazing range of 30°C. 
•The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.
•Gradient range 20°C to 70°C, maximum 30°C
•Decremental/incremental time and temperature

 

Technical Specification

Parameters TC-5000 TC-4000
0.5ml block capacity 60 60
0.2ml block capacity 96 96
384-well block Yes Yes
In-situ block Yes Yes
Temperature range 4°C to 99°C 4°C to 99°C
Gradient range 20°C to 70°C NA
Maximum gradient¹ 30°C (16°C for 384 block) NA
Maximum ramp rate² 3.0°C/sec 2.6°C/sec
Block uniformity at 50°C <±0.3°C <±0.3°C
Selectable heated lid temperature 100°C - 115°C 100°C - 115°C
Heated lid enable/disable Yes Yes
Over-temperature cut-out Yes Yes
Number of programs³ 50 80
Programming Touchscreen 4-line alphanumeric
Graphical display Real-Time Graph NA
Maximum hold time 99 hrs 18 hrs
Minimum hold time 1 sec 1 sec
Auto restart on power failure Yes Yes
Dimensions L x W x H 420 x 220 x 260mm 420 x 220 x 260
Voltage Dual voltage 230/115V 50-60Hz Dual voltage 230/115V 50-60Hz
Power 620W 620W
Shipping weight 14kg 13kg

 


Ordering Information

Product Code Description
FTC4/05 TC-4000 thermal cycler for 60 x 0.5ml microtubes, 120V / 230V
FTC4/H02 TC-4000 thermal cycler for 96 x 0.2ml microtubes or 96-wellplates, 120V / 230V
FTC4/F02 TC-4000 thermal cycler for 96 x 0.2ml microtubes or 96-well fully skirted plate, 120V / 230V
FTC4/FLAT TC-4000 thermal cycler for In-situ, 120V / 230V
FTC5/05 TC-5000 Gradient thermal cycler for 60 x 0.5ml microtubes, 120V / 230V
FTC5/H02 TC-5000 Gradient thermal cycler for 96 x 0.2ml microtubes or 96-well plate, 120V / 230V
FTC5/F02 TC-5000 Gradient thermal cycler for 96 x 0.2ml microtubes or 96-well fully skirted plate, 120V / 230V
FTC5/FLAT TC-5000 Gradient thermal cycler for In-situ (non-gradient), 120V / 230V


 

 

 

 

 

 

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC

Thermal cycler Techne TC-3000X non gradient and TC-3000G gradient

Thermal cycler Techne TC-3000X non gradient

TC-3000X

The new TC-3000X personal cycler is based on the unrivalled and reliable TC-3000. The new model is ideal for those laboratories that need just that little bit more, with its expanded sample capacity to accommodate 48 x 0.2ml tubes or for convenience, a 48-well plate.

• Increased sample capacity: The footprint remains the same, only 561cm2,but the capacity increases to 48 x 0.2ml or 30 x 0.5ml tubes
• Adjustable heated lid: The heated lid can now be adjusted for use with all PCR tubes, strip tubes or 48-well plates sealed with sealing mats
• Rapid heating rate: Utilising the latest Peltier technology the TC-3000X has a heating ramp rate of 3.3°C/sec and a 2.0°C/sec cooling rate
• Fast-track programming: Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple
• PC control & networking: The TC-3000X can be linked together with other Techne cyclers; up to 32 cyclers can be networked to one computer

 

Thermal cycler Techne TC-3000G gradient

 

The world's smallest gradient cycler, the TC-3000G will hold half a 96-well plate, in a horizontal format so that it offers 8 columns for annealing temperature optimisation and 6 rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over a 15°C gradient between the temperatures of 20 and 80°C.

All the features of the TC-3000 and TC-3000X plus...

• Gradient block: A gradient of up to 15°C between the temperatures of 20 and 80°C can be set, allowing any protocol to be optimised within a single experiment
• New software: Enables a gradient to be added to any temperature step of the program
• Gradient calculator: The gradient calculator function displays the temperature for each of the 8 columns, ensuring easy replication of thermal conditions

 

Technical Specification

Parameters TC-3000X TC-3000G
0.5ml block capacity 30 30
0.2ml block capacity 48 48
Temperature range 4°C to 99°C 4°C to 99°C
Maximum ramp rate 3.3°C/sec 3.3°C/sec
Gradient range NA 20°C to 80°C
Maximum gradient NA 15°C
Block uniformity at 50°C ±0.2°C ±0.2°C
Peltiers per block 6 6
selec table heatd lid temperature 100°C to 115°C 100°C to 115°C
Heated lid enable/disable Yes Yes
No. of programs 80 80
Programming display 4-line alphanumeric 4-line alphanumeric
Auto restart on power failure Yes Yes
Dimensions L x W x H 330 x 170 x 190mm 330 x 170 x 190mm
Voltage 230/115V/100V 50-60Hz 230/115V/100V 50-60Hz
Power 230W 230W
Shipping weight 6kg 6kg

 

 


Ordering Information

Product Code Description
FTC3X/02 TC-3000X thermal cycler for 48 x 0.2ml microtubes, 100V/115V/230V
FTC3X/05 TC-3000X thermal cycler for 30 x 0.5ml microtubes, 100V/115V/230V
FTC3G/02 TC-3000G gradient thermal cycler for 48 x 0.2ml microtubes, 100V/115V/230V
FTC3G/05 TC-3000G gradient thermal cycler for 30 x 0.5ml microtubes, 100V/115V/230V
FTC3X/02/B TC-3000X block for 48 x 0.2ml microtubes
FTC3X/05/B TC-3000X block for 30 x 0.5ml microtubes
FTC3G/02/B TC-3000G gradient block for 48 x 0.2ml microtubes
FTC3G/05/B TC-3000G gradient block for 30 x 0.5ml microtubes

 

 

 

 

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC

 

Thermal cycler Techne TC-3000 non gradient

 

The TC-3000 has been designed with research and teaching laboratories in mind, the TC-3000 offers the ultimate in low cost solutions where ease of use is high on the priority list. Experience in thermal cycler manufacturing since 1987, ensures that the TC-3000 provides guaranteed performance and quality results, every time.

• Space saving small footprint: at only 561cm2, the TC-3000 is even economical on space
• Rapid heating rate with a 3.6°C/sec heating rate and 2.0°C/sec cooling rate 
• Adjustable heated lid (100°C to 115°C), designed to reduce the risk of sample evaporation, can be enabled or disabled. The heated lid only comes on if the block is set above 35°C 
• Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple 
• The TC-3000 can be linked together with up to 32 cyclers can be networked to one computer
 

 

Technical Specification

Parameters TC-3000
0.5ml block capacity 20
0.2ml block capacity 25
Temperature range 4°C to 99°C
Maximum ramp rate 3.6°C/sec
Block uniformity at 50°C ±0.1°C
Peltiers per block 4
selec table heatd lid temperature 100°C to 115°C
Heated lid enable/disable Yes
No. of programs 80
Programming display 4-line alphanumeric
Auto restart on power failure Yes
Dimensions L x W x H 330 x 170 x 190mm
Voltage Dual voltage 230/115V 50-60Hz
Power 230W
Shipping weight 8kg

 

 


Ordering Information

Product Code Description
FTC3/02 TC-3000 thermal cycler for 25 x 0.2ml microtubes, 115V/230V
FTC3/05 TC-3000 thermal cycler for 20 x 0.5ml microtubes, 115V/230V
FTC3/02/B TC-3000 block for 25 x 0.2ml microtubes
FTC3/05/B TC-3000 block for 20 x 0.5ml microtubes

 

 

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

 

Techne TC-512 Thermal Cycler

Optimisation at a single touch

 

The Techne TC-512 is the only Gradient thermal cycler which enables you to optimise your experiments at the “touch of a screen.”

 

 Wide linear gradient
The most linear gradient cycler on the market, with an amazing range of 30°C. Protocols can be optimised in a single experiment.
 
TouchScreen
The Touchgene Gradient incorporates a large, 115 x 90mm, touch-sensitive screen that provides a unique user-friendly programming interface which is second to none.
 
Graphical display
The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment’s status.The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment’s status.
 
o Memory cards: SmartMedia memory cards provide an alternative to internal memory storage of your programs, offering security and flexibility.
 
o Gradient calculator: The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.

 

o 4-Fold control: Each block consists of 8 peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block. This ensures that the TC-512 exhibits the most accurately controlled linear gradient available*.
 
o High performance: A maximum heating rate of 3°C/Sec and block uniformity of ±0.4°C. With or without a gradient......no compromise!
 
o Versatile block format: The truly user-friendly interchangeable block system allows blocks to be interchanged in a matter of seconds without the need of any tools (60 x 0.5ml 96 x 0.2 ml, 3841 well, Insitu Block2).
 
o Proven track record: Having more than 13 years experience in thermal cycler design ensures that the Techne TC-512 encompasses reliability with all the specifications you could wish. These include: PC control and networking, decremental/incremental time and temperature, and defined pressure programmable heated lid.
 
o Intuitive programming: Easy to use software with free upgrades downloadable from the Techne website as new functionality is added.
 
o New Design Flexible Heated Lid: Defined pressure, high quality, programmable heated lid to accommodate a variety of consumables..
 

Graph showing the linearity of a 0.2ml Touchgene gradient cycler block in relation to the ideal linearity. The Techne block exhibits an almost perfect linearity with deviations occurring only at the block edges where the laws of thermodynamics prevent a perfect linearity form existing. This is due to Quad circuit technology.    
* at time of going to print
1. maximum gradient 16o on 384 well block
2. Insitu block available as non-gradient only 

SPECIFICATION
 

BLOCK TEMPERATURE

Gradient  range 20ºC to 70ºC
Maximum gradient 30ºC
Minimum gradient 1ºC
Number of temperature probes 4
Block temperature range 4ºC to 99ºC
Max. ramp rate* 3.0ºC/sec
Block uniformity without a gradient* <±0.4ºC
Column uniformity with a 10ºC gradient* <±0.3ºC
Temperature accuracy*  <±0.3ºC
Gradient accuracy* <±0.3ºC
Temperature set point adjustment 0.1ºC
Heated Lid
Selectable heated lid temperature  100ºC - 115ºC
Heated lid enable/disable Yes
Warm up time ambient (to 105ºC) 4 min
Over temperature cut out Yes
Regulated lid pressure Yes
Heated lid only comes on if block is set above 30º  
Programming
Password protection Yes
Incremental / decremental temperature Yes
Incremental / decremental temperature segment hold time Yes
Variable programmable ramp rate steps ±0.1ºC/sec
Programmable ramp rate resolution 0.1ºC
Gradient calculator Yes
Maximun programs internal memory 50#
Maximun programs memory card 500#
Program name capability Alphanumeric
Programming interface (115x90 mm screen) Touch Sensitive
Pause facility Yes
End of program alarm (can be disabled) Yes
Auto restart on power failure Yes
Run end time calculation Yes
Miscellaneous
Dimensions L x W x H (mm) 420 x 220 x 260
Net weight 11.5kg
Serial port RS 232 / RS 485 Yes
Connection to PC control program  Yes
Free software upgrades from website Yes
Power consumption 620 Watts
Ambient temprature range 5ºC to 40ºC
Relative humidity tolerance up to 95%
Frequency 50 - 60 Hz
Voltage 100v or 120v/230v 


*specifications given are typical values in ambient temperature of 20 ºC for non-gradient (unless stated).
#Actual numbers dependent on complexity of the programs. 

Ovens - Hybridisation - Techne
 

 

Hybrigene (HB-3D)

• Temperature range from 10°C above ambient to 80°C
• Can hold up to 16 mini or 4 large glass tubes
• Flexibility at an affordable price, offering excellent temperature accuracy and uniformity
• The Hybrigene is a compact, stackable alternative to the Hybridiser HB-1D - for when space is important
• Stack up to 3 ovens, saving on valuable laboratory space

HB-1D

• The famous Hybridiser HB-1D is compact, easy to use and provides complete protection from hybridisation hazards
• A temperature range of 10°C above ambient to 100°C
• High capacity which can hold up to 24 mini tubes or 6 unique large tubes
• Hybridisations can be performed with a minimal volume of 5ml of probe
• Adjustable feet to enable accurate leveling
• Unique double-glazed glass door; quiet and safe, providing durable protection

Technical Information

Model Hybrigene (HB-3D) HB-1D
Maximum glass tube capacity 16 mini tubes 24 mini tubes
Maximum temperature 80°C 100°C
Minimum temperature 10°C above ambient 10°C above ambient
Adjustable rotation speed 0, 5 to 20 rpm 0, 5 to 20 rpm
Adjustable rocking platform 5-20 or 15-60 opm 5-20 or 15-60 opm
Stability in chamber <1.0°C <1.0°C
Stability in tubes <±0.1°C <±0.1°C
Uniformity in chamber <±1.5°C <±1.5°C
Uniformity in tube <±0.5°C <±1.0°C
Temperature set point resolution 0.1°C 0.1°C
Absolute accuracy <±0.3°C <±0.3°C
Dimensions L x W x H (mm) 355 x 383 x 432 285 x 385 x 555
Voltage 230V, 50-60Hz 230V, 50-60Hz
Power 750W 750W
Shipping Weight 21kg 24kg

 

Techne Hybridisation Tube Accessories For The Hybridiser HB-1D And Hybrigene
 

Glass hybridisation tubes
reduces volumes, simplify washing, improve signals!


 

Large tube

 
  Small tube
(For use without carrier)
 
  • 3 sizes available:
    small & mini and unique
    large tubes
  • Durable:
    thick borosilicate glass
  • Easy pour:
    non drip, safe and convenient
  • Sealing Ring:
    “O” ring sealed end caps
    allow no leakage!
  • Broken thread on screw cap:
    easy opening with no vacuum problems
  • Tube Assemblies:
    Adaptors to hold multiple
    small glass tubes to
    increase capacity.
Mini tube assembly

 
  Small tube assembly
(Hybrigene only)
 
   
Hybridisation Accessories

Tube holders
Tube holders with clips, available to carry 16 x 15ml or
8 x 50 ml tubes. Ideal for a higher throughput of smaller samples.

 

 

Adjustable speed rocking platform
Fits on the base of the unit still allowing hybridisation tubes to be placed above. Enables membranes to be processed using a wave motion. Available in fast or slow speeds, which range from 5 to 60 oscillations per minute.

 

 

Static shelf 
Up to 2 (Hybrigene) or 3 (Hybridiser HB-1D) static stainless steel mesh shelves can be placed into the tube locators allowing the unit to be used as a simple non-motion incubator. Each shelf holds up to 4 kg.

 

 

Membrane separators 
Strong, re-usable porous membranes for placing between blots. Ensure even hybridisation when processing multiple hybridisation membranes in one tube. Available as packs of 5 (20x20 cm).

 

 

Hybridisation glass tube holder
Useful storage facility for tubes during membrane loading or when not in use. Carries up to 3 large and 3 small tubes.


8 x 50 ml tube holder


16 x 15 ml tube holder


Rocking platform


Static shelf 

Hybridisation glass tube holder

   
Hybridisation Tubes And Carriers  
Tube types  Part No.  Size (mm)
Diam. x L
Priced  Total
capacity
Description 
Hybrigene     
Large glass tube FHB16* 80 x 240 single 4 Glass tube with push on end caps
Large glass tube no cap FHB15 80 x 240 single 4 Glass tube with no end caps
Small glass tube FHB36 44 x 240 single 4 Glass tube with screw-on end cap,
tube has end plug
Mini glass tube assembly FHB41 32 x 205 pack of 4 4 x 4 Glass tubes with screw-on end cap,
requires carrier FHB61
  FHB61 tube carrier single   Carrier for tubes FHB41
Small glass tube assembly FHB71 44 x 220 single 4 x 3 Glass tube with screw-on end cap,
without end plug. Requires FHB70
  FHB70 tube carrier single   Carrier for tubes FHB71
  FHB72 assembly complete 4 x 3 Complete with carrier (FBH70)
and 3 small tubes (FHB71)
15ml tube holder F15ML4TH tube carrier single 4 x 16 Carrier with clips to hold 16 x 15 ml tubes
(approx. diameter 16.5mm)
50ml tube holder F50ML4TH tube carrier single 4 x 8 Carrier with clips to hold 8 x 50ml tubes
(approx. diameter 29mm)
Hybridiser HB-1D
Large glass tube FHB12* 80 x 200 single 6 Glass tube with push on end caps
Large tube no cap FHB11 80 x 200 single 6 Glass tube with no end caps
Small glass tube FHB32 44 x 200 single 6 Glass tube with screw-on end cap,
tube has end plug
Mini glass tube assemblies FHB41 32 x 205 pack of 4 6 x 4 Glass tubes with screw-on end cap,
requires FHB43
  FHB43 tube carrier single   Carrier for tubes FHB41
15ml tube holder F15ML1TH tube carrier single 6 x 16 Carrier with clips to hold 16 x 15 ml tubes
(approx diameter 16.5mm)
50ml tube holder F50ML1TH tube carrier single 4 x 8 Carrier with clips to hold 8 x 50ml tubes
(approx diameter 29mm)

*sealing rings supplied with these parts are suitable only for operation up to 70°C.
For operation up to 100°C, part number 61014588 x 2 should be ordered     

 

Application notes:

Hybridisation protocol tips 
Hybridisation techniques which involve the detection of membrane bound molecules are commonplace in most laboratories and include Northern (RNA), Southern (DNA) and Western (Protein) blot. These may come in the form of molecules transferred from a gel, slot or dot blots (where they are spatially placed on top of the membrane using a pipette or specialised manifold), or by colony/plaque adhesion. During the hybridisation process, while larger volumes of wash buffers are used, small volumes of probe and detection solutions are desired, all requiring a variety of temperatures. Below are some useful tips which improve the quality of your results.

Hybridisation vessels 
To optimise your results use specially designed hybridisation incubators with rotating bottles. These offer many advantages including: reduced reagent volume, even membrane hybridisation, multiple membranes/vessel, easy probe recovery, temperature accuracy and reproducibility, safe liquid transfer.

Multiple membranes 
When a large number of blots are to be hybridised with the same probe, time and reagents can be saved by placing them together. To do this, pre-warm the first reagent and place it in the pre-warmed incubation vessel before adding the membranes one by one, always ensuring that each one is fully coated before adding the next. To optimise probe: target accessibility use separation membranes between each blot.

Improving signal: noise ratios
One of the most difficult aspects of membrane hybridisation is to keep a clean particulate free blot with a low background, while still retaining a good signal:

  • Temperature: Chose the correct temperature. The easiest way to decrease the background during RNA and DNA blot hybridisation procedures is to increase the temperature at which the probe and initial post-hybridisation steps are carried out. As a guide, for a longer DNA probe (e.g. 200bp) start with 65°C and for an oligonucleotide probe try 50°C. For protein detection, some primary antibodies are more specific when hybridised overnight at 4°C rather than a few hours at room temperature.

 

  • Probes & antibodies: Take care to keep stocks of probes and detection reagents at the recommended temperatures. For non-radioactive techniques, re-using probe and antibody hybridisation solutions can lead to significant improvements of signal (store antibodies at 4°C and DNA at -20°C). When using antibodies, give the stock tubes a quick spin in a microcentrifuge and pipette from the top of the liquid to remove background causing precipitants.

 

  • Clean equipment: Cleanliness plays an important part in membrane hybridisations. Always wear gloves, handle blots with forceps and avoid dusty environments. For non-radioactive detection techniques, microwave the reaction vessels with some water in prior to re-use as this acts to remove residues which can cause serious background problems.

 

  • Buffers: Only use high quality reagents and ultra-pure water. When making up stocks of large volume buffers such as SSC, pass them through 0.45 µm filters to remove particulates. If using formamide, make sure it has been deionized first.

 

  • Crosslinking: Using a UV-Crosslinker  to irreversibly bind your nucleic acids to the membrane is faster and much more efficient than traditional baking and results in a higher signal.
     

Block heater Techne twin control dry requires 2 blocks

 

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

The innovative design accommodating two blocks with independent digital temperature controls. Each block can be set at different temperatures - ideal for multiple users or for applications where samples have to be transferred between two temperatures very quickly.

• Very accurate temperature control
• Digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 


DB-2TC


• Ideal for multiple users or applications
• Two blocks with independent temperature controls
• Can hold up to 2 aluminium insert blocks
• 4-digit setting with bright orange LED digital displays for fast and accurate setting of temperature
• Powerful heater for fast heat-up rate: 30°C to 100°C in just 19 minutes
• Temperature range from ambient to 100°C
• Temperature stability at 40°C: ±0.1°C

 

 

Technical Specification

 

Parameter DB-2TC
Temperature range Ambient to 100°C
Maximum no. of blocks 2
Temperature stability at 40°C ±0.1°C
Temperature setting Push button
Temperature display Orange 4 digit LED
Maximum variation between identical blocks at 40°C 0.2°C
Uniformity within block at 40°C ±0.1°C
Uniformity within block at 100°C ±0.1°C
Set point accuracy ±1°C
Heat up time: 30 to 37°C 6
Heat up time: 30 to 56°C 14
Heat up time: 30 to Maximum 19
RS232 option available No
Heater power 2 x 150W
Power supply 230V 50-60 Hz
Dimensions L x W x H (mm) 279 x 260 x 105
Shipping Weight 5kg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ordering Information

Product Code Description
FDB02DDTC DB-2TC ambient to 100°C, requires 2 lnsert blocks

 

 

 

Block inserts need to be purchased separately

 

Block heater Techne dry requires 4 blocks

 

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

• Very accurate temperature control
• Digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 


DB-4D


• Can hold up to 4 aluminium insert blocks or two 96-well plate blocks
• Bright orange LED digital display for fast and accurate setting of temperature
• Temperature range from ambient to 100°C
• Powerful heater for fast heat-up rate: 30°C to 100°C in just 15 minutes
• Interchangeable insert blocks to accommodate a variety of tubes
• Temperature stability at 40°C: ±0.1°C


 

Technical Specification
 

Parameter DB-4D
Temperature range Ambient to 100°C
Maximum no. of blocks 4
Temperature stability at 40°C ±0.1°C
Temperature setting Push button
Temperature display Orange 4 digit LED
Maximum variation between identical blocks @ 40°C 0.2°C
Uniformity within block at 40°C ±0.1°C
Uniformity within block at 100°C ±0.1°C
Set point accuracy ±1°C
Heat up time: 30 to 37°C 13
Heat up time: 30 to 56°C 11
Heat up time: 30 to Maximum 15
RS232 option available Yes
Heater power 600W
Power supply 230V 50-60 Hz
Dimensions L x W x H (mm) 356 x 260 x 105
Shipping Weight 7kg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ordering Information
 

Product Code Description
FDB04DD

DB-4D ambient to 100°C, requires 4 lnsert blocks

 

Block heater Techne dry requires 3 blocks

 

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

• Very accurate temperature control
• Analogue or digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 


DB-3


• The DB-3 is designed to hold up to 3 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Temperature range from ambient to 100°C
• Fast heat-up rate: 30°C to 100°C in just 18 minutes
• Temperature stability at 40°C: ± 0.05°C


DB-3A


• Temperature range from ambient to 200°C for higher temperature applications
• Can hold up to 3 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Powerful heater for fast heat-up rate: 30°C to 200°C in just 30 minutes
• Temperature stability at 40°C: ±0.1°C
 


DB-3D and DB-3DL


• Can hold up to 3 aluminium insert blocks or one 96-well plate block
• Bright orange LED digital display for fast and accurate setting of temperature
• DB-3DL temperature range from ambient to 100°C and DB-3D up to 200°C
• Powerful heater for fast heat-up rate: 30°C to 200°C in just 25 minutes
• Interchangeable insert blocks to accommodate a variety of tubes
• Temperature stability at 40°C: ± 0.1°C


 

Technical Specification
 

Parameter DB-3 DB-3A DB-3DL DB-3D
Temperature range Ambient to 100°C Ambient to 200°C Ambient to 100°C Ambient to 200°C
Maximum no. of blocks 3 3 3 3
Temperature stability @ 40°C ±0.05°C ±0.1°C ±0.1°C ±0.1°C
Temperature stability @ 100°C ±0.1°C ±0.15°C ±0.15°C ±0.15°C
Temperature setting Rotary knob Rotary knob Push Button Push Button
Temperature scale graduation 2°C 2°C No graduation No graduation
Temperature display NA NA Orange 4 digit LED Orange 4 digit LED
Maximum variation between identical blocks @ 40°C 0.2°C 0.2°C 0.2°C 0.2°C
Set point accuracy ±2°C ±2°C ±1°C ±1°C
Heat up time: 30 to 37°C 8 8 8 11
Heat up time: 30 to 56°C 12 12 9 15
Heat up time: 30 to Maximum 18 30 12 25
RS232 option available No No No Yes
Heater power 450W 450W 450W 450W
Power supply 230V 50-60Hz 230V 50-60Hz 230V 50-60Hz 230V 50-60Hz
Dimensions L x W x H (mm) 279x260x105 279x260x105 279x260x105 279x260x105
Shipping Weight 6kg 6kg 6kg 6kg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ordering Information
 

Product Code Description
FDB03OD DB-3 ambient to 100°C, requires 3 lnsert blocks
FDB03AD DB-3A ambient to 200°C, requires 3 lnsert blocks
FDB03LDD DB-3DL ambient to 100°C, requires 3 lnsert blocks
FDB03DD DB-3D ambient to 200°C, requires 3 lnsert blocks
FDB03DDR DB-3D + RS232 ambient to 200°C, requires 3 lnsert blocks


 

 

 

 

Block inserts need to be purchased separately

 

Block heater Techne dry requires 2 blocks


 

 

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

• Very accurate temperature control
• Analogue or digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 


DB-2A


• Small, light and compact footprint, economical price
• Can hold up to 2 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Temperature range from ambient to 100°C
• Fast heat-up rate: 30°C to 100°C in just 12 minutes
• Temperature stability at 40°C: ± 0.05°C


DB-2D and DB-2DH


• Bright orange LED digital display for fast and accurate setting of temperature
• RS232 connection available for PC control
• Can hold up to 2 aluminium insert blocks or one 96-well plate block
• Temperature range from ambient to 100°C or 200°C
• Fast heat-up rate 30°C to 100°C from just 12 minutes
• Temperature stability at 40°C of less than ± 0.1°C


 

 

Technical Specification
 

Parameter DB-2A DB-2D DB-2DH
Temperature range Ambient to 100°C Ambient to 100°C Ambient to 200°C
Maximum no. of blocks 2 2 2
Temperature stability @40°C ±0.05°C ±0.05°C ±0.1°C
Temperature stability @ 100°C ±0.1°C ±0.1°C ±0.15°C
Temperature setting Rotary graduated dial Push Button Push Button
Temperature scale graduation 2°C No graduation No graduation
Temperature display NA Orange 4 digit LED Orange 4 digit LED
Maximum variation between identical blocks @ 40°C 0.2°C 0.2°C 0.2°C
Set point accuracy ±2°C ±1°C ±1°C
Heat up time: 30 to 37°C 8 8 11
Heat up time: 30 to 56°C 9 9 15
Heat up time: 30 to Maximum 12 12 25
RS232 option available No Yes Yes
Heater power 300W 300W 300W
Power supply 230V 50-60 Hz 230V 50-60 Hz 230V 50-60 Hz
Dimensions L x W x H (mm) 202 x 260 x 105 202 x 260 x 105 202 x 260 x 105
Shipping Weight 4kg 5kg 5kg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ordering Information
 

Product Code Description
FDB02AD DB-2A ambient to 100°C, requires 2 lnsert blocks
FDB02DD DB-2D ambient to 100°C, requires 2 lnsert blocks
FDB02HDD DB-2DH ambient to 200°C, requires 2 lnsert blocks
FDB02DDR DB-2D + RS232 ambient to 100°C, requires 2 lnsert blocks

 

 

 

 

Block inserts need to be purchased separately

 

Techne TC-312

Thermal Cycler Accessories

Small, low cost
Out of the worlds best selling thermal cyclers, the TC-312 is the smallest, lowest cost unit on the market. It is available with 25 x 0.2 ml or 20 x 0.5 ml microtube interchangeable block formats.

Fast-track programming with free software upgrades
With modern, intuitive programming and ready-to-go templates, creating even the most complicated protocols is simple.

As new functionality is added to the software, updates can be downloaded for free from the Techne website.

Reliable, high performance
Experience in thermal cycler manufacturing since 1987, ensures that the TC-312 provides both guaranteed performance and quality results, everytime.

Portable Dual Voltage
This Compact unit is not only light, but has a variable voltage selector so you can take it with you anywhere in the world!
 


PC control Software FREE

SPECIFICATION

TEMPERATURE

Temperature range°C 4ºC to 99ºC

PROGRAMMING

 
Number of programs* 99 (3 segment programs)
Alnumeric Key Pad Yes
Program naming capability Alphanumeric
Program password Protection Yes
Display Large 4 line Alphanumeric
Maximum number of segments/loop* 80
Maximum number of repeat cycles per program * 99
End of program alarm Yes (can be disabled)
Pause facility Yes
Incremental/Decremental timing & Temperature Yes
Auto Restart on power failure Yes
Run end time calculation Yes
Variable programmable ramp rate steps ± 0.1ºC/Sec
Serial port RS 232 socket & PC connection Yes
Voltage selection switch (230 V/120V) Yes
Dimensions LxWxH (mm) 330 x 170 x 190
Footprint 561 cm2

NET WEIGHT 6kg

*dependent on complexity of the programs
BLOCK TEMPERATURE:
Block temperature range 4ºC to 99ºC
Heating ramp rate* 3.6ºC/Sec.
Cooling ramp rate 0.2 ml block* 2.0ºC/Sec.
Cooling ramp rate 0.5 ml block* 1.8ºC/Sec.
Uniformity at 50 ºC* 0.2ºC
Uniformity at 72 ºC* 0.4ºC
Uniformity at 95 C* 1.0ºC
Accuracy at 50 ºC* ± 0.2ºC
Accuracy at 72 ºC* ± 0.4ºC
Accuracy at 95 ºC* ± 0.3ºC
Temperature set point adjustment 0.1ºC
End of program cooling below ambient YES
* All specifications given are typical values under ambient temperature of 20ºC. More details are available upon request.

 

  Key Features:

o

Unparalleled block uniformity: ±0.1ºC at 50 ºC, ensuring optimal reproducibility

o

Fastest heating rate: up to 3.6ºC/Sec heating rate and 2.0ºC/Sec cooling rate

o

Smallest footprint yet: at only 561 cm2, the TC-312 is even economical on space

o

PC control & networking: The TC-312 can be networked together with other Techne cyclers allowing up to 32 cyclers to be connected to one PC.

o

Password protection: Protect your program against unwanted modifications.

o

Heated lid: The selectable temperature range heated lid (+100ºC to +115ºC) is sprung to fit a variety of height tubes and can be enabled or disable

o

Full 2 year warranty
 

Technical notes (REF FTGN-01)

To provide our customers with more accurate and meaningful information, Techne are making detailed block specifications available for their thermal cyclers of the new millennium. As all users are aware, the most important temperature to be accurate is that of the primer: template annealing. For this reason we are giving temperature specifications at a variety of points so that you know what is really happening. As a result, using the most up-to-date technology we are able to bring you temperature uniformity across the TC-312 block within ±0.1ºC*.

As Techne specialises in temperature calibration equipment, we are also able to make available to the user the temperature accuracy of the block. This value gives the average temperature spread which may occur around the absolute "real" temperature the block is set to. The TC-312 achieves an amazing accuracy within ±0.2ºC The specifications at key temperatures can be seen in the Block Temperature Specification table.

*Uniformity is the typical variation in temperature which may occur across the block under ambient conditions of 20ºC.