Gradient Master
This is biocomp's premier gradient forming instrument.
in as little as 40 sec, you can produce 6 identical, linear gradients using a patented process we call tilted tube rotation. The process involves layering the two end point solutions (i. E. 5% and 30% sucrose) directly in the centrifuge tube, capping it and placing it in a special tube holder with a magnetic base (magnabase™) that adheres to a steep plate on the instrument. Two keystrokes later, the gradient master takes over and tilts the tube to the proper angle, rotates it for a designated time and returns it to its original vertical position.
the introduction of the model 107 is the latest step in the evolution of this remarkable instrument. It has several new features that distinguish it from previous models. First, its memory contains all the run parameters for all the many different gradients we have developed for our customers. To see the list, select the rotor (ie sw41) you are using and then scroll through a list of the gradients organized by % and solute. To simplify things further, the “last” key on the keyboard recalls the last gradient run, so 2 keystrokes are all that are needed to duplicate a run.
second, the unit is now internet programmable. You can download new software yourself as upgrades become available. The gradient catalog is also upgradable so users can benefit from our ever-expanding set of run parameters. Solutes used so far include sucrose, glycerol, optiprep®, nycodenz®, ficoll®, percoll®, metrizamide, renografin®, nacl, cscl, naac, kcl. Like the old saying, the gradient master has never met a solute it didn’t like.
the gradient master is also a patented, intelligent roller blot device that can perform all the blocking, blotting and reaction steps with unerring accuracy and reproducibility. Using our patented magnabase™ bottles which come in a variety of sizes to fit every blot without overlap, you can reduce your reagent use by 90% while obtaining superb blots.
if you are serious about your gradient work, there is no competition for the gradient master. In nearly a thousand labs around the world for 18 years, it has become the gradient former of choice because of its simplicity, incredible speed and durability. features:the base unit includes a backlit lcd display, control knob and "smart" keypad for data entry and control. A 6" steel plate is tilted and turned by two stepper motors. The kit includes mac and pc serial cables for programming the unit, a bubble level, two 4” cannula for layering of gradient solutions and a selection of allen wrenches for maintenance.
Part Code:
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Description
|
List Price
|
108-2 |
Gradient Master base unit with steel plate for MagnaBase tube holders & bottles. 240V version. This product requires at least one MagnaBase holder to make it functional. Please let us know what types and sizes of tubes you will be using and also the types of centrifugation you will be doing (zonal, isopycnic or both) so that we can quote for the relevant tube holders and caps. Alternatively, please choose from the list below. |
£4504
|
Popular gradient forming attachments |
105-925-R |
1" (25mm) MagnaBase holder, marker block & 6 rate zonal caps for SW28/SW32 rotors |
£390 |
105-925-I |
1" (25mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW28/SW32 rotors |
£390 |
105-925-IR |
1" (25mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW28/SW32 rotors |
£498 |
105-916-R |
5/8" (16mm) MagnaBase holder, marker block & 6 rate zonal caps for SW27.1, 28.1 rotors |
£387 |
105-916-I |
5/8" (16mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW27.1, 28.1 rotors |
£387 |
105-916-IR |
5/8" (16mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW27.1, 28.1 rotors |
£473 |
105-914A-R |
9/16" (14mm) MagnaBase holder, marker block & 6 rate zonal caps for SW41 and like rotors |
£387 |
105-914A-I |
9/16" (14mm) MagnaBase holder, marker block & 6 Long, 10mm Isopycnic caps for SW41 and like rotors |
£387 |
105-914A-IR |
9/16" (14mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW41 and like rotors |
£473 |
105-914B-R |
9/16" (14mm) MagnaBase holder, marker block & 6 rate zonal caps for SW40 and like rotors |
£387 |
105-914B-I |
9/16" (14mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW40 and like rotors |
£387 |
105-914B-IR |
9/16" (14mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW40 and like rotors |
£473 |
105-913-R |
1/2" (13mm) MagnaBase holder, marker block & 6 rate zonal caps for SW50.1, 65 rotors |
£387 |
105-913-I |
1/2" (13mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW50.1, 65 rotors |
£387 |
105-913-IR |
1/2" (13mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW50.1, 65 rotors |
£473 |
105-911A-R |
7/16" (11mm) MagnaBase holder, marker block & 6 rate zonal caps for SW60 rotor |
£387 |
105-911A-I |
7/16" (11mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW60 rotor |
£387 |
105-911A-IR |
7/16" (11mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW60 rotor |
£473 |
105-911B-R |
7/16" (11mm) MagnaBase holder, marker block & 6 rate zonal caps for TLS-55 rotor |
£387 |
105-911B-I |
7/16" (11mm) MagnaBase holder, marker block & 6 Isopycnic caps for TLS-55 rotor |
£387 |
105-911B-IR |
7/16" (11mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for TLS-55 rotor |
£473 |
105-911B |
7/16" (11mm) MagnaBase holder, marker block for TLS-55 rotor; caps are the same as the 105-911A |
£351 |
Gradient Making Equipment - Biocomp
|
The performance of its two elements, the Piston Gradient Fractinator™ and the Gradient Master™ is identical to the separate instruments.
Features: The Gradient Station combines all the great features of the Gradient Master and the Piston Gradient
Gradient Master - see product 108-2
This is biocomp's premier gradient forming instrument.
in as little as 40 sec, you can produce 6 identical, linear gradients using a patented process we call tilted tube rotation. The process involves layering the two end point solutions (i. E. 5% and 30% sucrose) directly in the centrifuge tube, capping it and placing it in a special tube holder with a magnetic base (magnabase™) that adheres to a steep plate on the instrument. Two keystrokes later, the gradient master takes over and tilts the tube to the proper angle, rotates it for a designated time and returns it to its original vertical position.
the introduction of the model 107 is the latest step in the evolution of this remarkable instrument. It has several new features that distinguish it from previous models. First, its memory contains all the run parameters for all the many different gradients we have developed for our customers. To see the list, select the rotor (ie sw41) you are using and then scroll through a list of the gradients organized by % and solute. To simplify things further, the “last” key on the keyboard recalls the last gradient run, so 2 keystrokes are all that are needed to duplicate a run.
second, the unit is now internet programmable. You can download new software yourself as upgrades become available. The gradient catalog is also upgradable so users can benefit from our ever-expanding set of run parameters. Solutes used so far include sucrose, glycerol, optiprep®, nycodenz®, ficoll®, percoll®, metrizamide, renografin®, nacl, cscl, naac, kcl. Like the old saying, the gradient master has never met a solute it didn’t like.
the gradient master is also a patented, intelligent roller blot device that can perform all the blocking, blotting and reaction steps with unerring accuracy and reproducibility. Using our patented magnabase™ bottles which come in a variety of sizes to fit every blot without overlap, you can reduce your reagent use by 90% while obtaining superb blots.
if you are serious about your gradient work, there is no competition for the gradient master. In nearly a thousand labs around the world for 18 years, it has become the gradient former of choice because of its simplicity, incredible speed and durability. features:the base unit includes a backlit lcd display, control knob and "smart" keypad for data entry and control. A 6" steel plate is tilted and turned by two stepper motors. The kit includes mac and pc serial cables for programming the unit, a bubble level, two 4” cannula for layering of gradient solutions and a selection of allen wrenches for maintenance.
Gradient Fractionator - see product 152-002
Developed by a scientist who routinely does 6-30 gradient experiments, this device has more performance-enhancing features than all competing designs combined. You can recover slices from the gradient as small as 10 microns thick (300 micron fractions are more typical), visualize bands of particles as small as ribosomes with the naked eye, fractionate manually to recover visible bands or automatically to produce perfect gradient profiles, and rinse and blow dry your sample tubing after each fraction, eliminating cross-contamination between fractions.
the most important technical advance is our patented “trumpet tip”™, an inverted tapered piston cone that gradually compresses bands of particles from horizontal discs into thin vertical columns. This advance greatly reduces the resolution-killing laminar capillary flow that tends to pull sample from a teardrop shaped area directly in front of the needle or cone used by our competitors.
Another significant improvement is the readout of distance (0. 00 mm) from the meniscus down, so you know exactly where you are sampling in the tube.
the results are nothing short of spectacular. In a recent paper, jardine & coombs (1998) resolved 4 distinct peaks of phage t4 capsids between 300s - 350s where previous reports fractionated by needle puncture had shown only one broad peak. One of the new particles was a new intermediate in head assembly that demonstrated that capsid expansion followed the beginning of dna packaging.
The combination of the gradient master and the piston gradient fractionator offer a quantum leap in reproducibility and resolution. Call today for details. features: • visible light scattering band visualization system for particles as small as viruses and ribosomes.
Part Code:
|
Description
|
List Price
|
153-002 |
Gradient station Biocomp 240V version base unit with user manual - 4" plate - Combines the Gradient Fractionator and the Gradient Master in a single instrument |
£16542 |
Fractionator Tube Holders |
151-125 |
Fractionator tube holder and piston tip for 1" (25mm) x 3 1/2" centrifuge tubes (SW28/SW32) With a box of 50 1" x 3 1/2" PolyClear™ open top SW28 Ultracentrifuge tubes - retainer cap and cursor - spare piston tip and seal |
£2121 |
151-116 |
Fractionator tube holder and piston tip for 5/8" (16mm) x 4" centrifuge tubes (SW28.1 etc.) With a box of 50 5/8" x 4" PolyClear™ open top SW28.1 |
£2121 |
151-114A |
Fractionator tube holder and piston tip for 9/16" (14mm) x 3 1/2" centrifuge tubes (SW41/TH641) With a box of 50 9/16" x 3 1/2" PolyClear™ open top SW41 Ultracentrifuge tubes - retainer cap and cursor - spare piston tip and seal |
£2121 |
151-114B |
Fractionator tube holder and piston tip for 9/16" (14mm) x 3 3/4" centrifuge tubes (SW40) With a box of 50 9/16" x 3 3/4" PolyClear™ open top SW40 Ultracentrifuge tubes - retainer cap and cursor - 3 spare piston tips and seals |
£2121 |
151-113 |
Fractionator tube holder and piston tip for 1/2" (13mm) x 2" centrifuge tubes (SW50.1 etc.) With a box of 50 1/2" x 2" PolyClear™ open top SW50.1 Ultracentrifuge tubes - retainer cap and cursor - 3 spare piston tips and seals |
£2121 |
151-111A |
Fractionator tube holder - piston and piston tip for 7/16" x 2 3/8" centrifuge tubes. With a box of 50 7/16" x 2 3/8" PolyClear™ SW60 Ultracentrifuge tubes - retainer cap and cursor - spare valve assembly - piston tip and seal |
£3179 |
151-111B |
Fractionator tube holder - piston and piston tip for 7/16" x 1 3/8" centrifuge tubes. With a box of 50 7/16" x 1 3/8" PolyClear™ TLS-55 Ultracentrifuge tubes - retainer cap and cursor - spare valve assembly - piston tip and seal |
£3179 |
151-111B-T |
Fractionator tube holder ONLY for 7/16" x 1 3/8" centrifuge tubes. Includes a box of 50 7/16" x 1 3/8" PolyClear™ TLS-55 Ultracentrifuge tubes. Uses same piston - valve assembly - cursor - tips and seals as 151-111A |
£1608 |
Popular gradient forming attachments |
105-925-R |
1" (25mm) MagnaBase holder, marker block & 6 rate zonal caps for SW28/SW32 rotors |
£390 |
105-925-I |
1" (25mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW28/SW32 rotors |
£390 |
105-925-IR |
1" (25mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW28/SW32 rotors |
£498 |
105-916-R |
5/8" (16mm) MagnaBase holder, marker block & 6 rate zonal caps for SW27.1, 28.1 rotors |
£387 |
105-916-I |
5/8" (16mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW27.1, 28.1 rotors |
£387 |
105-916-IR |
5/8" (16mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW27.1, 28.1 rotors |
£473 |
105-914A-R |
9/16" (14mm) MagnaBase holder, marker block & 6 rate zonal caps for SW41 and like rotors |
£387 |
105-914A-I |
9/16" (14mm) MagnaBase holder, marker block & 6 Long, 10mm Isopycnic caps for SW41 and like rotors |
£387 |
105-914A-IR |
9/16" (14mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW41 and like rotors |
£473 |
105-914B-R |
9/16" (14mm) MagnaBase holder, marker block & 6 rate zonal caps for SW40 and like rotors |
£387 |
105-914B-I |
9/16" (14mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW40 and like rotors |
£387 |
105-914B-IR |
9/16" (14mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW40 and like rotors |
£473 |
105-913-R |
1/2" (13mm) MagnaBase holder, marker block & 6 rate zonal caps for SW50.1, 65 rotors |
£387 |
105-913-I |
1/2" (13mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW50.1, 65 rotors |
£387 |
105-913-IR |
1/2" (13mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW50.1, 65 rotors |
£473 |
105-911A-R |
7/16" (11mm) MagnaBase holder, marker block & 6 rate zonal caps for SW60 rotor |
£387 |
105-911A-I |
7/16" (11mm) MagnaBase holder, marker block & 6 Isopycnic caps for SW60 rotor |
£387 |
105-911A-IR |
7/16" (11mm) MagnaBase holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for SW60 rotor |
£473 |
105-911B-R |
7/16" (11mm) MagnaBase holder, marker block & 6 rate zonal caps for TLS-55 rotor |
£387 |
105-911B-I |
7/16" (11mm) MagnaBase holder, marker block & 6 Isopycnic caps for TLS-55 rotor |
£387 |
105-911B-IR |
7/16" (11mm) MagnaBase™ holder, marker block, 6 Isopycnic caps and 6 rate zonal caps for TLS-55 rotor |
£473 |
105-911B |
7/16" (11mm) MagnaBase holder, marker block for TLS-55 rotor; caps are the same as the 105-911A |
£351 |
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