This is BioComp's
premier gradient forming instrument.
In as little as 40 sec, you can produce 6 identical, linear
gradients using a patented process we call tilted tube rotation. The
process involves layering the two end point solutions (i.e. 5% and 30%
sucrose) directly in the centrifuge tube, capping it and placing it in a
special tube holder with a magnetic base (MagnaBase™) that adheres to
a steep plate on the instrument. Two keystrokes later, the Gradient
Master takes over and tilts the tube to the proper angle, rotates it for
a designated time and returns it to its original vertical position.
The introduction of the model 107 is the latest step in the
evolution of this remarkable instrument. It has several new features
that distinguish it from previous models. First, its memory contains all
the run parameters for all the many different gradients we have
developed for our customers. To see the list, select the rotor (ie SW41)
you are using and then scroll through a list of the gradients organized
by % and solute. To simplify things further, the “LAST” key on the
keyboard recalls the last gradient run, so 2 keystrokes are all that are
needed to duplicate a run.
Second, the unit is now internet programmable. You can download
new software yourself as upgrades become available. The gradient catalog
is also upgradable so users can benefit from our ever-expanding set of
run parameters. Solutes used so far include sucrose, glycerol, Optiprep®,
Nycodenz®, Ficoll®, Percoll®, Metrizamide, Renografin®, NaCl, CsCl,
NaAc, KCl. Like the old saying, the Gradient Master has never met a
solute it didn’t like.
The Gradient Master is also a patented, intelligent roller blot
device that can perform all the blocking, blotting and reaction steps
with unerring accuracy and reproducibility. Using our patented MagnaBase™
bottles which come in a variety of sizes to fit every blot without
overlap, you can reduce your reagent use by 90% while obtaining superb
blots.
If you are serious about your gradient work, there is no
competition for the Gradient Master. In nearly a thousand labs around
the world for 18 years, it has become the gradient former of choice
because of its simplicity, incredible speed and durability.