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Precision 2000 

Automated Microplate Pipetting System


Overview


Precision 2000TM is an affordable, innovative solution for automated 96-/384-well microplate liquid handling. With its ability to perform virtually any routine liquid transfer, Precision 2000 replaces manual pipetting.

The configurable multi-station platform and powerful onboard software allow for customized applications. The unique XY transport design provides effortless 96- to 384-well plate transfers with the same pipette mechanism. Additionally, robotic plate handlers can be seamlessly interfaced to Precision 2000 via an available ActiveXTM component. Bio-Tek's proprietary pipette technology provides a reliable way to pick up and seal standard tips with controlled force. Precision 2000 solves the sealing issues associated with conventional pipettes.

Precision 2000 offers a variety of fluid transfer operations including:
  • Reagent addition
  • Mixing within a well
  • Serial dilutions
  • Rapid Dispense Mode
  • Plate replication
  • Sample transfer
    • Plate-to-plate (96 to 96, 384 to 384, 96 to 384)
    • Within a plate

Features


Hardware:

  • Pipette:
    Precision 2000's uniquely designed pipette is made up of 12 or 8 individual syringes and guarantees reliable tip pickup. Each syringe is free floating within the body of the pipette. When tips are picked, the syringes are free to move up to accommodate differences in height and radially to accommodate deviations from ideal position. Precision 2000 achieves the most reliable seal on all tips with equal force.
  • Work Surface:
    Precision 2000 comes equipped with a user configurable platform. A reagent trough, disposable tip container and up to six 96- or 384-well microplates can all be positioned where they best suit your application. The entire platform can be removed for easy cleaning. When pipette tips are used, a programmer can decide whether to return the tips to the source for reuse or discard in the waste vessel provided.
  • Robotic Compatibility:
    Robotic compatibility is a standard feature with Precision 2000. The configuration of the platform combined with its XY transport capability make Precision 2000 compatible with robotic plate handlers. An ActiveXTM package is available for simple integration.
  • Rapid Dispense Mode:
    Precision 2000 ensures fast, flexible and precise automated liquid delivery. Rapid Dispense Mode is made possible with an optional manifold. A separate positive displacement pump allows for the continuous rapid dispensing of reagent from any size reservoir to both 96- and 384-well microplates.
  • Safety:
    Precision 2000 optimizes safety in today's laboratory. As an automated pipetting system, Precision 2000 helps eliminate repetitive motion injuries. Its compact size makes Precision 2000 ideally suited for installation into a biological safety cabinet. When installed on the lab bench, an optional protective cabinet is also available to prevent aerosols. This protective cabinet shields the operator while Precision 2000 is running. An internal software safeguard is available at any point in the liquid transfer if a pause or program abort is necessary.

Software:

 

  • Pre-Defined Demo Programs, Program Creation Ability:
    Precision 2000 comes pre-installed with some of the most common pipetting routines, including reagent additions, serial dilutions and plate-to-plate transfers. The software is thoughtfully designed to enable the user to copy an existing demonstration protocol and simply modify it to meet specific requirements. Up to 80 program files can be stored in memory. The user has the ability to create, edit, copy and delete files from the external keypad with a 2 x 24 character display. Virtually any liquid transfer parameter can be customized. Configurable aspiration depth ensures that tips don't touch the bottom of wells while managing sensitive cell assays.
  • Validation, Printing, Lock Feature:
    Precision 2000 offers an automatic validation utility which ensures proper creation or modification of protocols before any liquid transfer can take place. This feature conserves time and expensive supplies. After a protocol is created or modified, parameters can easily be printed for an additional verification. A convenient lock feature is also available for security purposes.
  • QC Programs:
    Precision 2000 comes equipped with pre-programmed protocols for Installation Qualification, Operational Qualification and Performance Verification needs. Each instrument is factory tested for optimal precision and accuracy performance. Performance documentation accompanies each instrument, and tests can be easily duplicated by the user.

Models


Precision 2000 is available in several configurations. All configurations support 96- and 384-well microplates and are robot compatible.

Universal Precision 2000 (PRC384U)
Automated pipetting system with both 12- and 8-channel liquid transfer capabilities. Includes rapid dispense mode.

12-Channel Precision 2000 (PRC384/12)
Automated pipetting system with 12-channel liquid transfer capability and rapid dispense mode.

8-Channel Precision 2000 Plus (PRC384M)
Automated pipetting system with 8-channel liquid transfer capability and rapid dispense mode.

8-Channel Precision 2000 (PRC384)
Automated pipetting system with 8-channel liquid transfer capability.

Standard Accessories

 

  • 4 microplate holders
  • 3 pipette tip box/reagent trough holders
  • 4 50 ml reagent troughs
  • Parallel printer cable: 25-pin male to 36-pin male
  • Serial cable: null modem, 9-pin female to 25-pin female
  • Maintenance kit
  • Operator's manual

Optional Accessories

 

  • Protective aerosol cabinet
  • Precision 2000 pipette tips
  • Many additional labware adapters are available. Inquire for more details.
  • Precision PowerTM Software
    Precision PowerTM is Bio-Tek’s Windows PC software for complete Precision 2000 control. The graphical user interface simplifies program creation under Windows 95, 98, NT, 2000 and XP. Programs can either be created and run from the PC or downloaded and run from Precision 2000’s onboard microprocessor. Precision Power expands the instrument's dynamic capabilities with features such as a graphical simulation utility, nested looping, and the ability to replenish supplies mid run.

    Precision Power PC Requirements:
    • Intel Pentium processor/133 MHz (or compatible)
    • Microsoft Windows 95, 98, 2000, NT (Service Pack #6) or XP operating systems
    • 32MB of RAM minimum
    • 60MB of available hard drive space
    • CD ROM drive
    • SVGA color monitor (800x600 resolution with 256 colors)
    • Keyboard, mouse and serial ports
  • Precision Power ActiveX Programmer's package
  •  

     

    Specifications

    PERFORMANCE

    Dispensing precision
    < 2% when dispensing 100µl per well of deionized water with 0.1% Tween 20 with FD&C #1 blue dye.

    Dispensing accuracy
    < 2% when dispensing 100µl per well of deionized water with 0.1% Tween 20 with FD&C #1 blue dye.

    Throughput
    12-Channel Pipette: < 80 seconds to dispense 100 µl per well of fluid to all wells of a standard 96-well microplate.

    8-Channel Pipette: < 100 seconds to dispense 100µl per well of fluid to all wells of a standard 96-well microplate.

    12-Channel Manifold: < 40 seconds to dispense 50 µl per well of fluid to all wells of a standard 384-well microplate.

    8-Channel Manifold: < 50 seconds to dispense 50µl per well of fluid to all wells of a standard 384-well microplate.

    TECHNICAL

    Tips
    Accommodates Bio-Tek and other standard 50 to 250 µl pipette tips.

    Microplates
    Accommodates 96- and 384-well flat-, round- and V-bottom plates.

    Pipette
    12-channel, 10-120 µl per channel (PRC384U, PRC384/12)

    8-channel, 10-120 µl per channel (PRC384M, PRC384)

    Manifold
    12-channel, 20-9999 µl per channel (PRC384U, PRC384/12)

    8-channel, 20-9999 µl per channel.

    Supply bottle volume
    2 liters

    User interface
    External membrane keypad with 25 alphanumeric keys and a 2-line x 24-character LCD display.

    Optional PC software.



    Main voltage
    100 to 240 VAC, 50/60 Hz, < 0.7A

    Dimensions
    Instrument only
    15" D x 21" W x 16" H 
    (374mm D x 525mm W x 400mm H)

    Instrument with optional cabinet: 17.5" D x 23" W x 17.5" H 
    (435mm D x 575mm W x 435mm H)

    Weight
    Instrument only: 
    28 lbs. (12.7 kg)

    Instrument with optional cabinet:
    38 lbs. (17.2 kg)

    Operating conditions
    15 to 40oC (59 to 104oF)

    Relative humidity
    10 to 85% (non-condensing)

    Regulatory compliance
    For in vitro diagnostic use.
    All Bio-Tek microplate instrumentation is CE and ETL market.  For details on approvals and standards compliance, please contact Bio-Tek.

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