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Applied Biophysics ECIS Real Time Cell Growth Monitoring ECIS Model 9600 (6x16)
The impedance of small active electrodes in each well is measured in real time to record cell activity and response to agents. Like the ECIS™ 1600 instrument, the 6X16 mode monitors the simple (rather than complex) impedance of the ECIS electrodes at one of three fixed frequencies covering the range where impedance changes due to the presence of the cells are strongest. In addition to non-invasive measurements, the ECIS™ 9600 in the 6x16 mode can also be programmed to apply elevated fields for cell migration measurements via an automated wound-healing assay [PNAS 101: 1554-1559 (2004)]. High fields can also be used at shorter duration for electroporation of membrane-impermeable compounds into cells. The ECIS 9600 instrument sits beside the tissue culture incubator. Twelve leads connect the main instrument to the six array holder stations within the incubator space. A USB cable provides communication to a PC that controls all data acquisition, storage and analysis. In addition to the ECIS™ electronics and software, the system is supplied with a state-of-the-art PC/monitor (or laptop), color ink jet printer and an initial supply of electrode arrays. The user-friendly ECIS
software runs on a Windows XP Profession platform for all operations. In
addition to using the ECIS analysis and plotting programs, one can readily
convert data to text files for export to other data analysis systems. The (6X16) package allows one to have the equivalent of six ECIS™ 1600 instruments complete with elevated field applications but at great cost savings. Specifications Maximum number of
wells: 6x16 or 96 wells total |
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