Applied Biophysics ECIS Real Time
Cell Growth Monitoring
Chondrocyte Proliferation Data
Chondrocytes were harvested from
actively growing flasks and inoculated into ECIS wells to give an initial
surface concentration of approximately 1 x 104 cells per cm2. A
total of seven individual ECIS wells were inoculated and the impedance
measured every 10 minutes for over 150 hours. The arrays used from this work
were 8W10E with 10 small electrodes connected in parallel in each well. The
plot below shows the resistive portion of the impedance at 4000 Hz as a
function of time. One medium change took place at 100 hours post
inoculation.
The same data is summarized below, showing the time
course change in the average resistance of the seven wells minus the
starting resistance due to the cell-free electrode. Note that the medium
change at 100 hours causes a temporary departure from the otherwise
exponential appearance of the graph until confluence is achieved. The
subsequent change in impedance presumably comes about as cells crowd into
the layer resulting in more regions of cell-cell contact and a slight drop
in resistance. Using capacitance measurements at high frequency, this factor
can be essentially eliminated.
Tel
01759 301142 Fax 01759 301143
Wolf
Laboratories Limited, Colenso House, 1 Deans Lane, Pocklington, YORK,
YO42 2PX
If you
encounter a problem with any part of this website, please e-mail us at
sales@wolflabs.co.uk
